In order to extract and “immortalize” a viable HEK-293 stem cell sample which was eventually used in the Pfizer Quackcinne and other pharmaceutical products, the brain, heart and kidney of the baby had to be removed while STILL ALIVE WITHOUT the use of anesthesia 😧😭🤮🤬. The “293” in the name of the stem cell is a nod to the number of babies they ran through and grotesquely mutilated before attaining a viable sample that they could then deploy for commercial use in their nanotech-laced instrument of genocide more commonly known as the Kovid-19 vaccine. Granted this took place all the way back in 1973, but it doesn’t make it any less horrific or unethical and it makes you wonder just what else they do behind closed doors at big pharmaceutical companies.
On a side note, everyone be like dying like reeeally reeeally suddenly and out of nowherez. I wonder what it could duh be? Duhhhhhhh….. SADS: Sudden Adult Death Syndrome… 🤣😂🤣 fucking retards. | Australia Raises Alarm: Citizens Dying at ‘Incredibly High’ Rate. Officials in Australia are raising the alarm as the country’s excess deaths have soared to an “incredibly high” rate.
PFIZER VACCINES – EXPOSED – DISGUSTING REVELATION
Telegram: https://t.me/We_The_People_US/853
Source: CitizenWells.com
Covid vaccine HEK293 cells harvested from living 6 month old babies, Pfizer scientist identifies cell line, Biologist Pamela Acker explains, Taken alive by C section kidneys cut out without anesthesia
citizenwells.com
Covid vaccine HEK293 cells harvested from living 6 month old babies, Pfizer scientist identifies cell line, Biologist Pamela Acker explains, Taken alive by C section kidneys cut out without anesthesia
Covid vaccine HEK293 cells harvested from living 6 month old babies, Pfizer scientist identifies cell line, Biologist Pamela Acker explains, Taken alive by C section kidneys cut out without anesthesia
“Furthermore, when you get vaccinated you know that you are now subject to the risks of the vaccine,” he said. “If you don’t get vaccinated, you only have a chance of contracting Covid, and therefore a chance of assuming the risks of Covid—it’s not 100% certain you’ll get Covid just because you were not vaccinated.”…UCI Medical Ethics director
“Why are we vaccinating healthy adults when 81 percent of Covid-19 cases are mild and there is a 99 percent survival rate. Why are we testing vaccines on children who are minimally impacted by the disease?”…Citizen Wells
“I’m not afraid of blowing the whistle “because my faith lies in God and not man … You know, like what kind of person would I be if I knew all of this — this is evil at the highest level. You have the FDA [U.S. Food and Drug Administration], you have the [Centers for Disease Control and Prevention] CDC, that are both supposed to be protecting us, but they are under the government, and everything that we’ve done so far is unscientific.”..Jodi O’Malley registered nurse
The following was difficult to report.
It is satanic.
From GateWay Pundit October 13, 2021.
“Pfizer’s Nervousness About Its COVID Vaccine’s Origins Conceals a Horror Story
On October 6, with the help of James O’Keefe’s Project Veritas (PV), a whistleblower at Pfizer revealed, in leaked internal emails, that company executives have been, in PV’s words, “telling staff to be secretive about the use of human fetal tissue in laboratory testing of the COVID vaccine.””
“The details of HEK293’s brutal – and from all appearances, until very recently, largely undisclosed – origins explain the company’s deep discomfort. Contrary to nearly a half-century of misrepresentation and obfuscation, HEK293’s creation did NOT arise from an “abortion” as everyday people understand it.”
Biologist and author Pamela Acker has a master’s degree in biology and worked in the field of vaccine development.
From Crisis Magazine. Around minute 33:00 (of course)
“In the arena of formation in moral truth, the first is the letter from Bishop Athanatius Schnieder, which first appeared in Crisis on December 11, 2020. In regard to formation in scientific truth, the second is a recent interview with Pamela Acker, author of Vaccines: A Catholic Perspective from the Kolbe Center—a book I look forward to reading. It has been difficult for many faithful Catholics to inform their conscience with regard to the nuts-and-bolts science of how the vaccine industry makes use of aborted children’s bodies, because the information is not readily available, but this interview provides a great deal of help. However, its length might be prohibitive, and because its content is critical, I wish to share a few of the hard truths it contained, in light of the information otherwise made available to us.
In conversations with friends, I have noticed that many of us calm our consciences with certain facts. We know that the Pfizer and Moderna vaccines do not use any cells derived from abortion in the production process. That is, we know that we are not being directly injected with fetal cells or their engineered descendants (though this fact differs with other manufacturers). We hear that the abortion-derived cell lines were only used in testing, which should somehow comfort us, though it still means that the vaccines from which we seek to benefit depend on the involvement of abortion. We are told that the cell line used in testing came from one abortion, which took place decades ago. These things are all true, but they do not serve to inform us fully.
What we may not know follows. The most prominent cell line, called HEK 293, comes from an abortion performed in the 1970’s. It’s labeled 293 because that’s how many experimental attempts the researchers needed to get a working cell line. Therefore, though the abortion-to-experiment ratio is not precisely one-to-one, hundreds of abortions went into the project, even if they didn’t result in the working line.
HEK stands for human embryonic kidney. To harvest a viable embryonic kidney for this purpose, sufficiently healthy children old enough to have adequately-developed kidneys must be removed from the womb, alive, typically by cesarean section, and have their kidneys cut out. This must take place without anesthesia for the child, which would lessen the viability of the organs. Instead of being held, rocked, and comforted in the time intervening between their birth and their death, they have organs cut out of them alive.
There is no way that a spontaneous abortion could result in the cell line (as the kidneys cannot remain viable past the brief window in which they must be harvested) or that some brilliant researcher found a way for great good to come out of a rare tragedy by making use of a child’s body donated to science after it was aborted. The deliberate killing of an unwanted child (a little girl, in the case of HEK 293) took place in the tortuous manner it did precisely to obtain her organs for research. The harvest of her organs was the direct cause of her death, prior to which, she was a living child, outside the womb.”
More here:
https://parler.com/profile/Citizenwells/posts
Related
Physician Danice Hertz “horribly ill” and “incapacitated” after Pfizer COVID vaccination, Diagnosis of “mast cell disorder”, “They knew about these adverse reactions before the vaccines were released from the clinical trials” September 24, 2021In “CDC”
17 year old male gets 3 brain blood clots after Pfizer Covid vaccine mother regrets decision, 1043 reports of blood clotting disorders attributed to Pfizer, No Informed Consent!May 11, 2021In “Citizen News”
Source: https://stemcell-faq.com/is-hek293-a-stem-cell
Is Hek293 A Stem Cell?
stemcell-faq.com
The HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus
Adenoviridae
Adenoviruses are medium-sized, nonenveloped viruses with an icosahedral nucleocapsid containing a double stranded DNA genome. Their name derives from their initial isolation from human adenoids in 1953.
type 5 DNA. The adenoviral genes expressed in this cell line allow the cells to produce very high levels of recombinant proteins.Full Answer
What are the origins of HEK293 cells?
Origins of the HEK293 Cell Line. HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells.
What does HEK293 mean?
You can find the “additive” under the label HEK 293. It stands for “Human Embryonic Kidney cells, with 293 denoting that the HEK was from the 293rd experiment. HEK293 originally came from a healthy, electively aborted baby whose cells were then harvested and cloned.”
What are hek293-derived cell lines used for?
Another widespread use for HEK293-derived cell lines is the production of recombinant proteins that require either folding or post-translational modifications exclusive to mammalian cells.
What makes HEK293T cells attractive for plasmid replication?
Introducing the viral SV40 large T antigen has created HEK293T cells, enabling the replication of transfected plasmids with an SV40 origin of replication. This change has made HEK293T cells attractive for producing large amounts of recombinant proteins or viral particles, as high plasmid levels can be reached by transient transfections.
What type of cell is HEK293?
human embryonic kidney cellsHEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
Are HEK293 cells embryonic stem cells?
HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5).
Are 293T cells stem cells?
293T cells exhibit a cancer stem cell-like phenotype when cultured as 3D spheres and represent an important research tool for studying the molecular and biological mechanisms of cancer stem cells and for testing and developing novel targets for cancer therapy.
Where do HEK cells come from?
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.
What is HEK293 made of?
These cells were initially thought to originate from an endothelial, epithelial, or fibroblastic cell from the fetal kidney. However, recent evidence into the cellular characteristics of HEK293 cells has led to the suggestion that they may actually originate from a neuronal fetal kidney cell.
Are hek293t cancerous?
HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research. HEK 293 can be employed as a highly malignant tumor model as its tumorigenicity increases significantly in high-passage.
What is the difference between 293 and 293T cells?
The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production. 293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .
What have HEK 293 cells been used for?
The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.
What has HEK293 been used for?
HEK 293T, a variant of the HEK 293 cell line, has been recently developed. This cell derivative carries SV40 large T antigen, enabling these cells to yield recombinant proteins in plasmid vectors that contain SV40 promoters. Having said that, HEK 293T is widely used for viral particle production.
Are HEK cells safe?
When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses. A viral contamination event occurred with this cell line even after a number of precautions were taken.
Are HEK cells immortal?
HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
What does HEK stand for?
Also found in: Medical, Wikipedia. Acronym. Definition. HEK. Human Embryonic Kidney Cells.
What is the difference between 293 and 293T cells?
The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production. 293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .
What has HEK293 been used for?
The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.
How do HEK293 cells grow?
HEK293 are rounded cells that grow in suspension in cell culture, although initially they were an adherent cell line. HEK-293 cells should be grown in a complete SFMII growth medium supplemented with 4 mM L-glutamine. Flasks should be incubated at 37°C in 5% CO2 and HEK293 cell doubling time is approximately 34 hours.
Are HEK293 cells tumorigenic?
293 cells are tumorigenic, whereas acute changes of expression of the cancer-associated genes aggravate tumorigenicity by promoting chromosome instability. Importantly, the procedure of a stable empty vector transfection can also impact karyotype and phenotype.
Why are HEK293 cells used?
Originating from a female fetus, HEK293 cells are today among the most used mammalian cell lines for a wide range of applications because of their ease of transfection as well as culture. Despite stemming from kidney tissue, HEK293 cells – time and time again – exhibited properties of immature neurons. This curious behavior led to …
What is the application of HEK293?
One major application for HEK293 and its derived cell lines lies in fundamental research, as it allows for the detailed investigation of human proteins in a cellular background. For this, cells usually need to be transfected with a gene of interest. HEK293 cells are particularly easy to transfect and culture, …
What is HEK293T?
Both HEK293 and HEK293T represent adherent cell lines that form a stable connection with coated cell culture dishes. This limits their volume to a two-dimensional space. Therefore, HEK293F cells were selected to be cultured in suspension by adaptation, elevating them to the three-dimensional space and allowing for more efficient growth of high-density cell cultures for recombinant protein production in bioreactors. The cell line HEK293FT further merges benefits from HEK293T and HEK293F by introducing the SV40 large T antigen into HEK293F cells as well. Next to many additional derived cell lines, HEK293SG deserves to be mentioned. Stemming from the suspension-cultured HEK293S cell line, HEK293SG are designed to produce homogeneously glycosylated biopharmaceuticals by the deletion of MGAT, a key chokepoint of the glycosylation machinery.
What is a cell line from kidney cells?
Creating an immortalized cell line from kidney cells. Cell lines such as HEK293 cells are crucial for areas such as biotechnology, fundamental research into cell biology, and biomedical therapies of the future. Typically, the description of a ‘cell line’ is reserved for immortalized mammalian cells that can be continually cultured for a time …
What is the origin of HEK293?
As the method of immortalization influences the resulting cell line, the origin of a widely used cell line such as HEK293 can be illuminating for working with it. In 1973, Frank Graham (a postdoc in Alex van der Eb’s group in the Netherlands) cultured cells from human embryonic kidneys (HEK) to generate an immortalized cell line by transfecting DNA …
What is HEK 293 used for?
HEK 293 cells and their derived cell lines are widely used in research and biotechnology. Understanding their origin, capabilities, and limitations is essential for optimal results, which we discuss here.
What are the most important cell lines?
For those interested in fundamental research or drug testing, other human cell lines, such as the colorectal Caco-2 cell line or the breast cancer cell line MCF-7 could be valid options. For an alternative for recombinant protein production – especially for producing antibodies – Chinese hamster ovary (CHO) cells are among the most important cell lines. Finally, the propagation or production of viruses has to be performed in permissible cell lines for the respective virus, such as Madin-Darby Canine Kidney (MDCK) cells for influenza viruses.
What is HEK 293?
Human Embryonic Kidney 293 (HEK 293) cells are one of the most frequently used cell lines for biotechnology and research studies. These cells are popular because they are fast-growing and robust.
Where does HEK 293 come from?
Initially, HEK 293 was thought to derive from the fetal kidney’s endothelial, epithelial or fibroblastic cell. But evidence recently showed that these cells may have originated from neuronal fetal kidney cells.
How long does it take for a Hek 293 cell to double?
Their culturing requirement is generally uncomplicated. These cells double rapidly. Its doubling time is roughly about 34 to 36 hours.
What cells are used to explain the role of metformin?
In one study, HEK 293 cells were used to explain Metformin’s role in treating cancer. It was established in the study that Metformin helped in hindering cell migration and suppressing the expression of chemokine interleukin-8.
How did Graham make the immortalized cell line?
To produce an immortalized cell line, Graham transfected DNA from adenovirus type 5 into human chromosome 19 of the HEK cells. The integration of Ad5 into the genome of the cells led to the prevention of apoptosis, thus leading to the continuous production of the cell line. It took Frank Graham 293 experiments before achieving success, thus the name HEK 293.
How big is HEK 293?
The diameter of HEK 293 cells are normally around 11-15µm, depending on culture conditions. Those grown in monolayer culture will most likely emerge more flat with a larger diameter as compared to cells cultured in suspension.
Can kidney 293 cells be grown as adherent cells?
Human Embryonic Kidney 293 cells can be both grown as adherent or suspension but usually, these cells are cultured as an adherent monolayer.
What is the HEK293 cell line?
Thomas P. and Smart T. (2005) HEK293 cell line: A vehicle for the expression of recombinant proteins. J ournal of Pharmacological and Toxicological Methods, 51 :3, 187-200.
Why are Hek293 cells used?
HEK293 cells are used in cancer research, vaccine development, protein production, signal transduction and protein interaction studies, drug testing, and receptor de-orphanization, just to list a few!
How often do HEK293 cells divide?
There are many advantages of using HEK293 cells. They are a hardy, semi-adherent, low-maintenance cell line and divide rapidly, doubling about every 36 hours. They can be utilized for both transient and stable expression, can be cultured in suspension or as a monolayer, are easy to transfect (and can be transfected via a variety of methods), and are able to produce large amounts of recombinant proteins.
How often can you pass HEK293 cells?
Because they grow quite rapidly, you can passage your HEK293 cells every couple of days (in our lab, we typically passage on Mondays, Wednesdays and Fridays). Similar to other cell lines, it’s pretty standard procedure to split the cells when they are in log (growth) phase, meaning that they are not quite 100% confluent.
What is the diet of HEK293 cells?
Non-negotiables include a humidified incubator kept at 37°C with 5% CO 2 and a diet of high-glucose media such as Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with Fetal Bovine Serum (FBS). Culturing media can also contain broad-spectrum antibiotics such as Penicillin-Streptomycin-Glutamine in order to prevent common bacterial infections – but the use of antibiotics in routine cell culture can be detrimental to cells and results.
What is HEK293 used for?
The HEK293 cell line (and its derivatives) are incredibly useful in research and can be used for a variety of applications across different fields of research. If you need a cell line that can produce large amounts of recombinant proteins, or if you’re a cell culture beginner looking for a low-maintenance cell line, HEK293 might be for you!
What are the disadvantages of using human cells?
One key disadvantage of using any human cell line is that there is a risk of contamination with human-specific viruses – this risk can be mitigated, but it’s still an important consideration. Of course, the key to any successful cell culture is consistency and an excellent aseptic technique to avoid infection.
Where does the HEK293 cell come from?
Origins of the HEK293 Cell Line. HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
What type of cell line came from HEK293?
The type of kidney cell that the HEK293 cell line came arose from is unknown and it is difficult to conclusively characterize the cells post-transformation since adenovirus 5 could have significantly disrupted cell morphology and expression.
Why are HEK293 cells used?
HEK 293 cells are popular for their ease of growth and transfection ( HEK293 Transfection Kit ), making them a common cell culture in cancer research. In addition, high transfection efficiency of HEK293 cells produces exogenous proteins or viruses for pharmaceutical and biomedical research purposes.
What is the chromosome number of HEK293?
HEK293 is a hypotriploid cell line with modal chromosome number of 64 , which occurs in 30% of cells. Cells with higher ploidies occur less frequently at a rate of 4.2%. HEK293 cell lines display some cytogenetic instability.
How many kilobases are in the HEK cell?
The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells. The line was cultured by scientist Alex Van der Eb in the early 1970s at his lab at the University of Leiden, Holland.
What type of cells are derived from an embryonic kidney?
Although theoretically possible, most cells derived from an embryonic kidney would be endothelial, epithelial or fibroblast cells. Neuronal origin is suspected due to the presence of mRNA and gene products typically found in neurons. Today, HEK293 cells are frequently used in cell biology and biotechnology, second only to HeLa, …
Why do scientists prefer animal cell lines over human cell lines?
This concern, along with the known ability of animal cell lines to grow rapidly and yield a high amount of proteins, gave scientists reason to favor animal cell lines over human cell lines when producing recombinant proteins . However, advances in technology since then have allowed for an increase in human cell line use.
What are HEK293 cells used for?
They are widely used for the production of recombinant therapeutic proteins , vaccines, anticancer agents, and other clinically relevant drugs. HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the development of recombinant proteins or adenovirus productions, not least due to their human-like posttranslational modification of protein molecules to provide the desired biological activity. Secondly, they also exhibit high transfection efficiency yielding high-quality recombinant proteins. They are easy to maintain and express with high fidelity membrane proteins, such as ion channels and transporters, and thus are attractive for structural biology and electrophysiology studies. In this article, we review the literature on HEK293 cells regarding their origins but also stress their advancements into the different cell lines engineered and discuss some significant aspects which make them versatile systems for biopharmaceutical manufacturing, drug screening, structural biology research, and electrophysiology applications.
What is HEK293?
HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the development of recombinant proteins or adenovirus productions, not least due to their human-like posttranslational modification of protein molecules to provide the desired biological activity.
What is HEK293 cell line?
HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA to produce many recombinant proteins. HEK293s were originally isolated from human kidney cells by Dutch biologist Alex Van der Eb in 1973. These cells were grown in tissue culture from a female fetus. Later, they were transfected with sheared human adenovirus type 5DNA by Frank Graham, a postdoctoral fellow in Van der Eb’s laboratory. This cell line was named HEK293 because it was Frank’s 293 rd experiment.
What is a HEK293T?
HEK293t is a daughter cell line derived from the HEK293 original cell line that is transfected with a plasmid vector carrying SV40 origin of replication to produce a high amount of recombinant proteins. HEK293t is a human cell line that expresses a mutant version of the SV40 large T antigen. HEK293t was created in Michele Carlos’s laboratory at Stanford through the stable transfection of the HEK29E cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigen. It was originally referred to as 293/tsA1609neo. This is because the transfection used to create a cell line that confers neomycin resistance and expression of the tsA 1609 allele of SV40 large T antigen.
What are the Similarities BetweenHEK293 and HEK293t?
HEK293 and HEK293t are two cell lines that have been extensively used in cell biology research because of their propensity for transfection.
Can cell lines increase recombinant protein production?
These cell lines can greatly increase the amount of recombinant protein production.
What type of cell does Johnson and Johnson use?
To make their virus vector vaccine, Johnson & Johnson infects PER.C6 fetal cell line cells with adenovirus. All PER.C6 cells used to manufacture the Johnson & Johnson vaccine are descended from tissue taken from a 1985 elective abortion that also took place in the Netherlands.
What cell line is used to make Johnson and Johnson?
For the Johnson & Johnson vaccine, fetal cell lines were used in the production and manufacturing stage. To make the Johnson & Johnson vaccine, scientists infect PER.C6 fetal cell lines to grow the adenovirus vector. ( Learn more about how viral vector vaccines work .) All PER.C6 cells used to manufacture the Johnson & Johnson vaccine are descended from tissue taken from a 1985 abortion that took place in the Netherlands. This cell line is used because it is a well-studied industry standard for safe and reliable production of viral vector vaccines.
What is fetal cell line?
Fetal cell lines are cells that grow in a laboratory. They descend from cells taken from elective abortions in the 1970s and 1980s. Those individual cells from the 1970s and 1980s have since multiplied into many new cells over the past four or five decades, creating fetal cell lines. Current fetal cell lines are thousands …
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